Login
Diagnostic Electrophoresis
Science And Technology

Diagnostic Electrophoresis

Diagnostic electrophoresis is a fundamental laboratory technique widely used in molecular biology, clinical diagnostics, forensic science, and biomedical research. It plays a critical role in the separation, identification, and analysis of biomolecules such as DNA, RNA, and proteins. With the rapid advancement of molecular diagnostics and personalized medicine, proficiency in electrophoresis techniques has become essential for students, researchers, and healthcare professionals. This SAGE Summer School Certificate Course in Diagnostic Electrophoresis provides theoretical knowledge and hands-on laboratory training in agarose gel electrophoresis, SDS-PAGE, sample preparation, gel casting, staining methods, and result interpretation. The course is designed according to NEP-2020 guidelines and aims to enhance laboratory skills, research capabilities, and employability in biomedical and diagnostic laboratories.
Course ModeHybrid
Duration1 Week
Starts02 Jun 2026
Ends06 Jun 2026
Course Fee
₹1,000 /-
Registration Closed
About The Course

Overview

This SAGE Summer School Certificate Course in Diagnostic Electrophoresis provides theoretical knowledge and hands-on laboratory training in agarose gel electrophoresis, SDS-PAGE, sample preparation, gel casting, staining methods, and result interpretation. The course is designed according to NEP-2020 guidelines and aims to enhance laboratory skills, research capabilities, and employability in biomedical and diagnostic laboratories.

Course Objectives

• Introduce the principles and applications of electrophoresis in diagnostic laboratories.
• Develop understanding of biomolecule separation techniques.
• Provide hands-on training in agarose gel electrophoresis and protein electrophoresis.
• Demonstrate preparation of gels and electrophoresis buffers.
• Train participants in visualization and analysis of electrophoretic results.
• Enhance knowledge of molecular diagnostics and clinical applications.
• Develop competency in laboratory safety and electrophoresis troubleshooting.

Learning Goals

Course Outcomes

• Participants will understand the principles and types of electrophoresis.
• Prepare agarose gels and perform DNA electrophoresis.
• Conduct SDS-PAGE for protein separation.
• Analyze electrophoresis band patterns and interpret results.
• Apply electrophoresis techniques in clinical and research diagnostics.
• Demonstrate safe laboratory practices during electrophoresis experiments.

A certificate course in diagnostic electrophoresis typically involves developing skills in biomolecular separation, understanding electrophoretic techniques, and applying these methods in molecular diagnostics, genetic testing, and biomedical research.

Curriculum

Course / Modules

Module 1 (4 hrs): Introduction to Diagnostic Electrophoresis

Theory:
• Principles of electrophoresis
• History and development of electrophoresis techniques
• Types of electrophoresis
• Applications in diagnostics and research
• Safety precautions and laboratory guidelines
• Role of electrophoresis in molecular diagnostics

Module 2 (4 hrs): Basics of Biomolecules and Buffers

Theory:
• Structure of DNA, RNA, and proteins
• Charge and mobility of biomolecules
• Buffer systems used in electrophoresis

Practical:
• Preparation of electrophoresis buffers
• Preparation of sample loading solutions
• Understanding buffer composition

Module 3 (4 hrs): Agarose Gel Electrophoresis

Theory:
• Principle of agarose gel electrophoresis
• Gel concentration and pore size
• Visualization methods

Practical:
• Preparation of agarose gel
• Sample loading
• Running agarose gel electrophoresis
• Gel staining and visualization

Module 4 (4 hrs): Polyacrylamide Gel Electrophoresis (SDS-PAGE)

Theory:
• Principle of SDS-PAGE
• Protein separation methods
• Applications in protein diagnostics

Practical:
• Preparation of polyacrylamide gel
• Loading protein samples
• Running SDS-PAGE
• Observation of protein bands

Module 5 (4 hrs): Visualization and Data Analysis

Theory:
• Principles of gel staining techniques
• Interpretation of electrophoretic band patterns
• Documentation of results

Practical:
• Use of gel documentation systems
• Band measurement and comparison
• Recording experimental data

Module 6 (6 hrs): Clinical Applications and Troubleshooting

Theory:
• Applications in genetic and diagnostic testing
• Troubleshooting electrophoresis errors
• Quality control and laboratory reporting

Practical:
• Case-based analysis
• Troubleshooting experimental errors
• Assessment, applications, feedback and certificate distribution on Day 6

Learn From Experts

Our Trainers

Dr. N. Ganesh
Dr. N. Ganesh
Name                        &n...
Dr. Shikha Tiwari
Dr. Shikha Tiwari
DR. SHIKHA TIWARI Assistant Professor of Biotechnology| Head and Neck cancer research | +91-8305979899 [email protected], s...
Dr. C. S. Senthil Kumar
Dr. C. S. Senthil Kumar
Multidisciplinary scientist and educator with extensive experience in biomonitoring, cancer epidemiology, cytogenetics, and environmental health resea...
Need Help?

Frequently Asked Questions

1. What is the duration of the Certificate Course in Diagnostic Electrophoresis?
The course duration is 1 week, scheduled from 02 June 2026 to 06 June 2026, with daily sessions conducted in theory and practical format.

2. What practical techniques will be covered in this course?
Participants will receive hands-on training in agarose gel electrophoresis, SDS-PAGE (protein electrophoresis), gel casting, buffer preparation, staining methods, and electrophoretic result interpretation.

3. Who is eligible to enroll in this course?
This course is suitable for students and professionals from biotechnology, microbiology, molecular biology, pharmacy, life sciences, and related healthcare or research fields.

4. What mode will the course be conducted in?
The course will be conducted in offline / hybrid mode at the Molecular Biology & Biotechnology Laboratory and Smart Classroom of the university campus.

5. Will participants receive a certificate after completing the course?
Yes, participants who successfully complete the training sessions and assessment will receive a certificate in diagnostic electrophoresis at the end of the course.