Overview
This SAGE Summer School Certificate Course in Diagnostic Electrophoresis provides theoretical knowledge and hands-on laboratory training in agarose gel electrophoresis, SDS-PAGE, sample preparation, gel casting, staining methods, and result interpretation. The course is designed according to NEP-2020 guidelines and aims to enhance laboratory skills, research capabilities, and employability in biomedical and diagnostic laboratories.
Course Objectives
• Introduce the principles and applications of electrophoresis in diagnostic laboratories.
• Develop understanding of biomolecule separation techniques.
• Provide hands-on training in agarose gel electrophoresis and protein electrophoresis.
• Demonstrate preparation of gels and electrophoresis buffers.
• Train participants in visualization and analysis of electrophoretic results.
• Enhance knowledge of molecular diagnostics and clinical applications.
• Develop competency in laboratory safety and electrophoresis troubleshooting.
Our Trainers
Dr. N. Ganesh
Name : Dr.N.Ganesh
Designation : Dean (Clinical Research & Research Consultancy)
Department Affiliation : Administration, Sanjeev Agarwal Global Educational (SAGE) University, Bhopal (M.P)
Education Qualification : PhD Anatomy (Medicine Faculty 2002)
MSc. Genetics, Diploma Human Nutrition (Alison, Ireland, UK 2021), Diploma Allopathy (Council of Indian Paramedical & Vocational Faculty 2019), Diploma Computer application, Certificate course & Observership in Clinical Genetics, Cancer Genetics, Industrial Microbiology, Counselling, Aromatherapy & Clinical Andrology).
Experience in SAGE : 4 months 19 days
Experience outside SAGE : 28 Years as a Consultant Oncogenetics & Sr.
Scientist
Work Area & Expertise : Diagnostic Human Genetics, Cancer Genetics,Immunogenetics,
Pedigree charting, Genetic Counselling, Tumor research, In – vitro Human and Animal Tissues culture, Bone marrow Culture, Toxicology, Experimental Pharmacology, Dermatoglyphic, Human Anatomy, Clinical Biochemistry, Clinical Microbiology, Mutation Research, Bio-statistic, Clinical Trial, Pre-clinical Trial, Laboratory Design, Textile Design & Fine Art.
Research Credentials
Publications : 169 published, 6 communicated
Research Citation Score : 68704
H - index : 131
i10 - index : 864
Books/Book Chapters : 2 Books, 2 Chapters, 1 book under process.
IPR : 2 & 1 Filed
Projects : 22 completed (Rs. 4,35,28,328.00) & 2 Submitted (Rs.70,63,000.00)
PhD Guide : 22 (Awarded) & 4 (Submitted)
Awards : 52 (National & International), 89 (Best Scientific
Paper) & 72 (Best poster)
Health & Cancer Awareness : 27300
Dr. Shikha Tiwari
DR. SHIKHA TIWARI
Assistant Professor of Biotechnology| Head and Neck cancer research |
+91-8305979899 [email protected],
[email protected] https://orcid.org/0000-0001-8871-9842 Bhopal, India
SUMMARY Biological Research Scientist with over 6 years of experience integrating molecular biology techniques and cancer research. Demonstrated ability to optimize laboratory operations and deliver comprehensive publications aligned with strategic objectives and translational approach, with a passion for advancing cancer treatment.
EXPERIENCE Assistant Professor Institute of Advanced Research, Gandhinagar 05/2024 - 11/2024 Gujarat Conduct academic lectures and practical sessions for Biotechnology Students. Explore the role of microRNA in oral cancer diagnosis and therapeutic advancements Women Scientist All India Institute of Medical Sciences Bhopal 08/2021 - 04/2024 Bhopal, India Established the MicroRNA dysregulation role in oral cancer development and progression. Successfully investigated the role of single nucleotide polymorphism in oral cancer predisposition. Optimized tissue culture conditions, resulting in a consistent 20% improvement in viability for cryopreserved cells. Provided critical input in team meetings, shaping the direction of research and subsequent studies. Senior Research Fellow CIAE 06/2019 - 07/2021 Bhopal, India Contributed to the development of a novel volatiles for the storage of horticulture commodity. Assisted in the validation of e-nose Prepared detailed method files for GC-MS and optimize VOCs absorption with SPME fibers.
EDUCATION Ph.D. Biological science and engineering Maulana Azad National Institute of Technology 03/2012 - 04/2016 Bhopal, India Master of Science in Biotechnology Krishna Institute of Medical Sciences University 07/2008 - 06/2010 Karad, India
LANGUAGES Hindi Native English Proficient
KEY ACHIEVEMENTS Publication in reputed SCI indexed journals Principal author of the research publications in peer reviewed journals. From the total 17 publications, 10 are published in SCI indexed journals. One book edited"Diagnostic, Prognostic, and Therapeutic Role of MicroRNAs in Head and Neck Cancer" Elsevier. Active member of American association of cancer research Describe what you did and the impact it had. Research Grant Recipient Secured a 47 lakhs research grant from ICMRDHR
SKILLS Cell Culture Protocols Technical SOPs Assay Development Transfection optimization Data analysis
COURSES 'R' workshop Received certification on R software for expression analysis and processing Online Course on Research Ethics Committee Completed a rigorous and informative course on making and implementation of ethical research committee
Dr. C. S. Senthil Kumar
Multidisciplinary scientist and educator with extensive experience in biomonitoring, cancer epidemiology, cytogenetics, and environmental health research. Proven track record in academic teaching, research project management, grant writing, and publication of high-impact research in molecular and cellular biology.
EXPERIENCE
Scientist / Research Coordinator / Academic Leader
Served in multiple academic and research roles including Scientist, Research Fellow, Academic Advisor, Vice-Principal, Head of Biotechnology & Microbiology Departments, Assistant Professor, and Lecturer.
- Conducted interdisciplinary research in environmental exposure and cancer susceptibility
- Guided students and managed academic and research programs
- Conducted cytogenetic, immunological, and molecular biology studies
- Published multiple peer-reviewed research articles
- Managed research documentation, grant proposals, and academic administration
EDUCATION
Ph.D. Biotechnology
Rajiv Gandhi Proudyogiki Vishwavidyalaya (RGPV), Bhopal
Research at Jawaharlal Nehru Cancer Hospital & Research Centre
M.Phil. Biotechnology
Bharathidasan University
M.Sc. Biotechnology
Periyar University
B.Sc. Microbiology
Periyar University
KEY ACHIEVEMENTS
- 31+ peer-reviewed publications with international citations
- Recipient of CSIR Senior Research Fellowship
- Excellence in Dental Research Award (2024)
- Excellence in Research Award (2022)
- Multiple government and industry-funded research projects
SKILLS
Technical Skills:
Cytogenetics, Molecular Biology, Immunology, Cell Culture, Microbiology, Toxicology
Professional Skills:
Research Management, Data Analysis, Scientific Writing, Teaching, Grant Writing
Modules
Module 1 (4 hrs): Introduction to Diagnostic Electrophoresis
Theory:
• Principles of electrophoresis
• History and development of electrophoresis techniques
• Types of electrophoresis
• Applications in diagnostics and research
• Safety precautions and laboratory guidelines
• Role of electrophoresis in molecular diagnostics
Module 2 (4 hrs): Basics of Biomolecules and Buffers
Theory:
• Structure of DNA, RNA, and proteins
• Charge and mobility of biomolecules
• Buffer systems used in electrophoresis
Practical:
• Preparation of electrophoresis buffers
• Preparation of sample loading solutions
• Understanding buffer composition
Module 3 (4 hrs): Agarose Gel Electrophoresis
Theory:
• Principle of agarose gel electrophoresis
• Gel concentration and pore size
• Visualization methods
Practical:
• Preparation of agarose gel
• Sample loading
• Running agarose gel electrophoresis
• Gel staining and visualization
Module 4 (4 hrs): Polyacrylamide Gel Electrophoresis (SDS-PAGE)
Theory:
• Principle of SDS-PAGE
• Protein separation methods
• Applications in protein diagnostics
Practical:
• Preparation of polyacrylamide gel
• Loading protein samples
• Running SDS-PAGE
• Observation of protein bands
Module 5 (4 hrs): Visualization and Data Analysis
Theory:
• Principles of gel staining techniques
• Interpretation of electrophoretic band patterns
• Documentation of results
Practical:
• Use of gel documentation systems
• Band measurement and comparison
• Recording experimental data
Module 6 (6 hrs): Clinical Applications and Troubleshooting
Theory:
• Applications in genetic and diagnostic testing
• Troubleshooting electrophoresis errors
• Quality control and laboratory reporting
Practical:
• Case-based analysis
• Troubleshooting experimental errors
• Assessment, applications, feedback and certificate distribution on Day 6
Outcomes
• Participants will understand the principles and types of electrophoresis.
• Prepare agarose gels and perform DNA electrophoresis.
• Conduct SDS-PAGE for protein separation.
• Analyze electrophoresis band patterns and interpret results.
• Apply electrophoresis techniques in clinical and research diagnostics.
• Demonstrate safe laboratory practices during electrophoresis experiments.
Certificate course in Diagnostic Electrophoresis typically involves developing skills in biomolecular separation, understanding electrophoretic techniques, and applying these methods in molecular diagnostics, genetic testing, and biomedical research.
FAQs
1. What is the duration of the Certificate Course in Diagnostic Electrophoresis?
The course duration is 1 week, scheduled from 02 June 2026 to 06 June 2026, with daily sessions conducted in theory and practical format.
2. What practical techniques will be covered in this course?
Participants will receive hands-on training in agarose gel electrophoresis, SDS-PAGE (protein electrophoresis), gel casting, buffer preparation, staining methods, and electrophoretic result interpretation.
3. Who is eligible to enroll in this course?
This course is suitable for students and professionals from Biotechnology, Microbiology, Molecular Biology, Pharmacy, Life Sciences, and related healthcare or research fields.
4. What mode will the course be conducted in?
The course will be conducted in Offline / Hybrid mode at the Molecular Biology & Biotechnology Laboratory and Smart Classroom of the university campus.
5. Will participants receive a certificate after completing the course?
Yes, participants who successfully complete the training sessions and assessment will receive a Certificate in Diagnostic Electrophoresis at the end of the course.
