Overview
This SAGE Summer School Certificate Course in Clinical Microbiology provides both theoretical knowledge and hands-on laboratory experience in microscopy, staining, microbial culture techniques, biochemical identification, antimicrobial susceptibility testing, and laboratory reporting procedures. The course is aligned with NEP-2020 guidelines and designed to enhance laboratory competency, research skills, and employability among students and healthcare professionals.
Course Objectives
- Introduce fundamental concepts of clinical microbiology and infectious diseases.
- Develop understanding of microbial classification and laboratory safety protocols.
- Provide hands-on training in microscopy and staining techniques such as Gram staining and acid-fast staining.
- Train participants in microbial culture, isolation, and identification methods.
- Demonstrate antimicrobial susceptibility testing using the Kirby-Bauer method.
- Enhance skills in laboratory diagnosis, quality control, and reporting of microbial infections.
- Promote research-oriented thinking and laboratory competency.
Outcomes
- The participant will understand principles and applications of clinical microbiology.
- Perform staining techniques, including Gram and acid-fast staining.
- Prepare culture media and perform microbial isolation techniques.
- Identify microorganisms using biochemical and morphological characteristics.
- Conduct antimicrobial sensitivity testing and interpret results.
- Apply microbiological knowledge in diagnostic and research settings.
Modules
Module 1 (2 hrs): Introduction to Clinical Microbiology
Theory:
• History and scope of Clinical Microbiology
• Classification of microorganisms
• Role of microbes in human diseases
• Biosafety levels and sterilization methods
• Laboratory safety and waste disposal
• Importance of Clinical Microbiology in healthcare
Module 2 (3 hrs): Microscopy & Staining Techniques
Theory:
• Principles of microscopy
• Types of microscopes used in microbiology
• Principles of staining techniques
Practical:
• Preparation of microbial smears
• Gram staining technique
• Acid-fast staining technique
• Microscopic observation and interpretation
Module 3 (2 hrs): Culture Techniques
Theory:
• Types of culture media
• Principles of microbial growth
• Sterilization methods
Practical:
• Preparation of culture media
• Inoculation techniques
• Streak plate method
• Aseptic handling of samples
Module 4 (4 hrs): Identification of Microorganisms
Theory:
• Morphological identification
• Colony characteristics
• Biochemical identification methods
Practical:
• Observation of colony morphology
• Biochemical testing procedures
• Recording identification results
Module 5 (5 hrs): Antimicrobial Sensitivity Testing
Theory:
• Principles of antimicrobial susceptibility testing
• Antibiotic resistance mechanisms
• Kirby-Bauer disc diffusion method
Practical:
• Preparation of inoculum
• Antibiotic disc placement
• Measurement of inhibition zones
• Interpretation of results
Module 6 (2 hrs): Clinical Applications & Laboratory Reporting
Theory:
• Sample collection and transport
• Diagnostic microbiology workflow
• Quality control procedures
• Laboratory reporting standards
Practical:
• Processing clinical samples
• Recording observations
• Preparation of laboratory reports
• Assessment, applications, feedback and certificate distribution on Day 6
Our Trainers
Dr. N. Ganesh
Dr. Shikha Tiwari
Dr. Arun Kumar Sharma
Dr. N. Ganesh
Dr. N Ganesh, FICER, (Dean Clinical Research & Research Consultancy, Sanjeev Agrawal Global University Bhopal)
EX- Consultant Oncogenetics, Head & Senior Scientist, Dept. of Research, Jawaharlal Nehru Cancer Hospital & Research Centre, since 1996.
He was awarded Ph.D. in Anatomy (Medicine) from Gandhi Medical College, Bhopal in 2002. He got trained from AIIMS New Delhi (Clinical & Molecular Genetics, Research Methodology), TATA Memorial Mumbai (Cancer Genetic), St. Johns Medical College Bangalore (Clinical Genetic), Kidwai Institute of Oncology Bangalore (Cancer Genetic), Kasturba Medical College Manipal (Radiation Biology), South West Foundation for Biomedical Research, San Antonio, USA in association with Anthropological Society of India (Molecular Genetic) & Beckman Coulter USA/India (Immunophenotyping).
He discovered active constituents against Skin Melanoma from Water Hyacinth (Jal Kumbhi). He also formulated herbal tea without sugar and tea leaves to reduce side effects of radiation and chemotherapy. I got patent for his formulation (Radiation Protector) extracted from Alpinia (Wild Zinger).
He am the first in the World to develop in-vivo Orthotopic Colon Tumor and metastasis Colon Tumor model in mice for targeted anti-cancer drug delivery.
He had an experience of working as Member Secretary of Institutional Ethics Committee for JNCHRC, Bhopal from 2000 and 2016. He is a Chairman of Institutional Ethics Committee of People’s Dental Academic since 2015.
He has completed 21 clinical and preclinical trials. Three ongoing. These projects were funded by ICMR, DST, DBT, CSIR, MPCOST, MPBTC, Elli Lilly, Cadila, Novartis, Johnson & Johnson, and Apothecaries etc.
He had published 134 scientific publications with a total impact factor of 210.11. He has presented more than 108 scientific papers & 157 posters at International & National Conference. Received 89 best paper and 72 best poster awards. Invited as Guest Speaker/Resource Person 840.
He guided 62 Ph.D & 300 Post graduate Dissertation Thesis. He is a registered Ph.D. Guide in Genetics, Biotechnology, Zoology, Life Sciences, and Pharmacology & Medicinal Chemistry from 7 National Universities.
He has received numerous International & National awards including Humboldt Kolleg Young Scientist appreciation Award (2003 Manipal, Udipi, Karnataka), State Best Scientist (Cancer Research 2013), VISWA (BHEL Society) award for Scientific contribution to state and Nation 2012, UGC sponsored Best visiting fellow Award in Pharmacology, Panjab University, Chandigarh, Punjab twice 2010 and 2013. Received Kadimey Millat Award for his contribution in the field of Cancer genetic diagnosis and Cancer Research in MP & CG. Eminent Scientist Award for Orthotopic tumor model by Rajeev Gandhi Group of Institutions (2014), Best Social worker Award in the field of Cancer awareness at Bundelkhand Vigyan Mela by MPCOST (2014). “Visiting Fellow Award” for best visiting faculty in Pharmaceutical Sciences of Panjab University, Chandigarh, Punjab. (2015), Excellence of service in the field of Cancer at Mandla Vigyan Mela by MPCOST (2015). “Award of Appreciation” for the contribution in the field of Cancer Awareness by Bhopal Mela Samiti (2016) and “Award of Appreciation” for tobacco cessation programme by People’s Group, Bhopal, (2017). “Outstanding Achievement Award” for the extra ordinary contribution in serving the society by BHEL education society Bhopal (2018). “ Gaurav Samman” for the untiring contribution in the field of Cancer Awareness by Parakaram Jansevi Sansthaan, Jago Bharat Abhiyan and Jago Bharat Chatr Sangathan of India, (2018). Sr. Scientist Award – ACAEE -2018. Outstanding Achievement Award (2019) by Utsav Mela Samiti, Bhopal for cancer awareness. “You Make a Difference award” (2020) by JNCHRC, Bhopal for outstanding performance throughout the tenure of service of 23 years at JNCHRC, Bhopal. Received “Award of Appreciation” for talk on “Cancer Awareness and Anticancer Diet Awareness: Myth to Fact” organized by UIPS, Panjab University (2020). Received "Healthcare Research Award for Genetic Oncologist of the Year" at “Asia Healthcare Summit & Awards 2020” organized by Asia Today Research & Media. MJF LION A V Mahajan (North Bhopal) Award for the outstanding services in the field of “Genetic diagnosis and anti cancer diet awareness” 2020. India Star Book of Record Awarded “India Star Personality Award 2020” for the appreciable work in the field of Anatomy. International Association of LIONS Club Awarded Dr.Ganesh for selfless services to the society 2020. At Constitutional Club of India, New Delhi by National Achievers Recognition Forum received National award for Cancer Awareness 2021. Institute of Scholars Research Excellence Award for the quality of work in academic and research, 2021. Outstanding work in the field of Cancer Genetics & Male Infertility awarded by; Intellectual Peoples Foundation, 2021. Received Pride of India Award- 2022 in recognition of sterling Merit Excellent Performance and outstanding contribution for the progress of the nation & worldwide at Constitutional Club of India, New Delhi by National Achievers Recognition Forum, 1st December, 2022. Received “Excellence in Oncogenetics Award” in recognition of sterling Merit Excellent Performance and Out-standing contribution since 1996 in Central India in the field of Clinical Genetics and Oncogenetics in Bangkok Thailand, on 8th & 9th December, 2022 at an International Conference organized by “PRISAL- Pharmaceutical Royal International Society” & Co-hosted by “TIPA- Thai Industrial Pharmacist association” on ‘Expanding Multi-Disciplinary Approaches for Healthcare & Well-being’ at Ambassador Hotel, Bangkok Thailand.
He is a life member in following societies: Indian Society of Human Genetics, Bhopal Haematology Society, Fellow Member - FICER (International Congress of Environmental Research), Society for Biosafety of India, Indian Fertility Society & The Cytometry Society of India
I had organized “Cancer Awareness Camps” on a regular basis and so far completed 2023 “Cancer and Anticancer Awareness Talks & Camps” throughout Bhopal and nearby places like Vidisha, Khurai, Raisen, Sagar, Rewa, Hoshangabad, Itarsi, Chindwara, Seoni Binaganj, Guna, Mandi Bamora, Sehore, Indore, Chatterpur, Damoh, Khajraho, Piperiya, Shuhagpur, Sarangpur, Panchmari and other remote villages of Madhya Pradesh, Chattisgarh, Punjab, Haryana, Jharkhand, Bihar, Jammu & Kashmir, Tamil Nadu, Kerala, Maharashtra, Gujrat, Utter Pradesh, Telangna, Andhra Pradesh, Pudochery, Karnataka, Chandigarh . The camps and talk were conducted Live and Virtual. He also conducts awareness talk on running trains and other public places.
He authored an International books on “Dermo Follicular Proliferation”- VDM Muller Publication, Germany & “Cytogenetic Variations in Bhopal Gas Tragedy Survivors” – Lambert Academic.
He is the only specialist in Diagnostic Clinical Genetics, Cancer Genetics, Genetic counselling in the entire Madhya Pradesh State and Chhattisgarh with special reference to Leukemia, Lymphoma, Multiple myeloma, Male infertility, Heritable diseases, Recurrent abortion, Sickle Cell Anaemia, Thalassemia.
He designed Animal house (27 Nation Wide) and Biotechnology Lab (07 Nation Wide).
In addition to scientific background, Dr.Ganesh also received many more awards in Painting, Rangoli, Collage, Clay Modelling & Classical Dance.
He visited Stockholm, Sweden to attend the investigator’s meeting for an International project Drug Trial on Locally Advanced Breast Cancer. Mauritius as a guest speaker in an “International conference on Environmental Research. He is also visited Dundee, Scotland to deliver a talk ‘Innovation in tumor biology and Role of biomarkers in time since death’.
Dr. Shikha Tiwari
DR. SHIKHA TIWARI
Assistant Professor of Biotechnology| Head and Neck cancer research |
+91-8305979899 [email protected],
[email protected] https://orcid.org/0000-0001-8871-9842 Bhopal, India
SUMMARY Biological Research Scientist with over 6 years of experience integrating molecular biology techniques and cancer research. Demonstrated ability to optimize laboratory operations and deliver comprehensive publications aligned with strategic objectives and translational approach, with a passion for advancing cancer treatment.
EXPERIENCE Assistant Professor Institute of Advanced Research, Gandhinagar 05/2024 - 11/2024 Gujarat Conduct academic lectures and practical sessions for Biotechnology Students. Explore the role of microRNA in oral cancer diagnosis and therapeutic advancements Women Scientist All India Institute of Medical Sciences Bhopal 08/2021 - 04/2024 Bhopal, India Established the MicroRNA dysregulation role in oral cancer development and progression. Successfully investigated the role of single nucleotide polymorphism in oral cancer predisposition. Optimized tissue culture conditions, resulting in a consistent 20% improvement in viability for cryopreserved cells. Provided critical input in team meetings, shaping the direction of research and subsequent studies. Senior Research Fellow CIAE 06/2019 - 07/2021 Bhopal, India Contributed to the development of a novel volatiles for the storage of horticulture commodity. Assisted in the validation of e-nose Prepared detailed method files for GC-MS and optimize VOCs absorption with SPME fibers.
EDUCATION Ph.D. Biological science and engineering Maulana Azad National Institute of Technology 03/2012 - 04/2016 Bhopal, India Master of Science in Biotechnology Krishna Institute of Medical Sciences University 07/2008 - 06/2010 Karad, India
LANGUAGES Hindi Native English Proficient
KEY ACHIEVEMENTS Publication in reputed SCI indexed journals Principal author of the research publications in peer reviewed journals. From the total 17 publications, 10 are published in SCI indexed journals. One book edited"Diagnostic, Prognostic, and Therapeutic Role of MicroRNAs in Head and Neck Cancer" Elsevier. Active member of American association of cancer research Describe what you did and the impact it had. Research Grant Recipient Secured a 47 lakhs research grant from ICMRDHR
SKILLS Cell Culture Protocols Technical SOPs Assay Development Transfection optimization Data analysis
COURSES 'R' workshop Received certification on R software for expression analysis and processing Online Course on Research Ethics Committee Completed a rigorous and informative course on making and implementation of ethical research committee
Dr. Arun Kumar Sharma
Ph.D.-qualified Microbiologist with over 12 years of experience in tuberculosis diagnostics, molecular diagnostics, biosafety systems, and laboratory quality management under the National TB Elimination Programme (NTEP). Experienced in TB culture, drug susceptibility testing, external quality assessment, and multi-center diagnostic validation studies with strong involvement in national TB surveillance and laboratory strengthening programs.
EXPERIENCE
Consultant Microbiologist (NRL) & Deputy Quality Manager (NABL)
ICMR – Bhopal Memorial Hospital & Research Centre (BMHRC)
06/2014 – Present | Bhopal, India
- Lead technical operations for tuberculosis diagnostics under NTEP
- Implemented and revised SOPs for TB laboratories
- Managed External Quality Assessment (EQA) programs
- Ensured biosafety compliance and BSL-III laboratory management
- Coordinated laboratory performance monitoring and reporting
Lecturer – Microbiology
Delhi Institute of Professional Studies
12/2011 – 05/2014 | New Delhi
- Delivered lectures on Microbiology, Immunology, Virology, and Parasitology
- Guided practical training for M.Sc and B.Sc students
Microbiology Research Associate
National Institute of Tuberculosis & Respiratory Diseases (NITRD)
2012 – 2014 | New Delhi
- Performed PCR, DNA extraction, and TB diagnostic assays
- Conducted solid and liquid TB cultures and DST
Clinical Microbiologist
Metropolis Healthcare Laboratory
02/2012 – 05/2012
Laboratory Technologist
Fatima Hospital, Mau (U.P.)
07/2008 – 06/2009
EDUCATION
Ph.D. in Microbiology
Mansarovar Global University, Sehore
2020 – 2025
M.Sc. Clinical Microbiology
Punjab Technical University, Jalandhar
2009 – 2011
Diploma in Medical Laboratory Technology (DMLT)
Christian Medical Association of India, New Delhi
2006 – 2008
B.Sc. (Biology)
V.B.S.P University, Jaunpur
LANGUAGES
Hindi — Native
English — Proficient
KEY ACHIEVEMENTS
- Co-Principal Investigator in ICMR Mega TB Project
- Published research articles in reputed microbiology journals
- Contributed to development of Biosafety Manual for Tuberculosis Laboratories (NTEP)
- Conducted training programs on TB diagnostics and biosafety
SKILLS
- Molecular Diagnostics (PCR, LPA, CBNAAT)
- Mycobacterial Culture & Drug Susceptibility Testing
- Laboratory Quality Management (NABL)
- External Quality Assessment (EQA)
- Biosafety & BSL-III Laboratory Management
- Data Analysis & Scientific Reporting
FAQs
1. Who can apply for the Certificate Course in Clinical Microbiology?
Students from life sciences, biotechnology, microbiology, pharmacy, medical, and allied healthcare fields, as well as interested researchers and healthcare professionals, can apply for this course.
2. What is the duration and schedule of the course?
The course duration is 1 week, scheduled from 15 May 2026 to 21 May 2026, with daily sessions from 10:00 AM to 4:00 PM.
3. Will the course include practical laboratory training?
Yes, the course provides hands-on laboratory training, including microscopy, Gram staining, acid-fast staining, microbial culture, biochemical identification, and antimicrobial sensitivity testing using the Kirby-Bauer method.
4. What mode will the course be conducted in?
The course will be conducted in hybrid mode (offline and online) at the Microbiology Laboratory and Smart Classroom of the university campus.
5. Will participants receive a certificate after completion?
Yes, participants who successfully complete the training, practical sessions, and assessment will receive a certificate in clinical microbiology at the end of the course.
