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Certificate Course in Mutation Research.
Science And Technology

Certificate Course in Mutation Research.

Mutation research is a core component of modern biological and biomedical sciences. This SWS certificate course provides theoretical foundations and hands-on laboratory training in cytotoxicity and genotoxicity assays such as Trypan Blue, MTT, and Micronucleus assays, aligned with NEP-2020 and industry requirements.
Course ModeHybrid
Duration1 Week
Starts12 Feb 2026
Ends18 Feb 2026
Course Fee
₹1,000 /-
Registration Closed
About The Course

Overview

The Certificate Course in Mutation Research is a focused, short-term program designed to introduce participants to the fundamental concepts and experimental approaches involved in the study of genetic mutations. The course covers key topics such as DNA damage, mutagens, mutation mechanisms, cell cycle alterations, and mutation-based assays, with strong emphasis on practical understanding and laboratory relevance. Through an integration of theory and hands-on learning, the program equips students with essential skills required for research in genetics, toxicology, cancer biology, and biomedical sciences. This course is ideal for students and early-career researchers seeking foundational training in mutation analysis and experimental genetics.

  • Introduce concepts of Cell, Mutation, Mutagenesis, and DNA damage.
  • Preparation of Hematological, Cytological & Histopathological Smear.
  • Explain principles of cytotoxicity and genotoxicity testing.
  • Provide hands-on training in Trypan Blue, MTT and Micronucleus assays.
  • Develop skills in data analysis and interpretation.
  • Enhance research competence and employability.
  • The participant will learn molecular basis and significance of mutations.
  • Trypan Blue & MT assay for cell viability.
  • Conduct MTT assay and calculate IC50 values.
  • Execute Micronucleus assay for genotoxicity assessment.
  • Analyze and interpret experimental mutation research data.
Learning Goals

Course Outcomes

Certificate course in mutation research typically involve developing skills in interpreting genetic variants, understanding the causes and mechanisms of mutations, and applying this knowledge to specific fields like cancer research, Pharma-toxicology or plant breeding. 

Curriculum

Course / Modules

Module 1 (4 hrs): Introduction to Cell Biology & Mutation Research

  • Definition and classification of mutations
  • Spontaneous vs induced mutations
  • Physical, chemical, and biological mutagens
  • DNA damage and repair mechanisms
  • Importance of mutation research in cancer biology and toxicology
  • Ethical and biosafety considerations

Module 2 (4 hrs): Cell Identification.

Differentiation between hematological, Cytological & Histopathological Smear/Section.

Practical:

  • Preparation of blood smear
  • Preparation buccal smear
  •  Preparation of Microbial smear
  • Staining (Hematological, Cytological & Histopathological Smear)

Module 3 (4 hrs): Cell Culture and Cytotoxicity Concepts

Theory:

  • Basics of cell culture techniques
  • Cell viability and cytotoxicity assays
  • Principles of dye exclusion methods

Practical:

  • Introduction to laboratory equipment
  • Aseptic handling of cells
  • Preparation of reagents

 

Module 4 (4 hrs): Trypan Blue Exclusion Assay

Theory

  • Principle of Trypan Blue Assay

Practical:

  • Cell counting using hemocytometer
  • Assessment of live and dead cells
  • Calculation of cell viability percentage
  • Data recording and interpretation

Module 5 (4 hrs): MTT Assay – Principle, Execution, and Data Analysis

Theory:

  • Principle of MTT assay
  • Role of mitochondrial activity in cell viability
  • Applications in cytotoxicity screening

Practical:

  • Preparation of MTT reagent
  • Treatment of cells with test compounds
  • Measurement of absorbance using ELISA reader
  • IC₅₀ determination
  • Graphical representation of results
  • Statistical interpretation
  • Limitations and precautions of MTT assay

 

 

 

Module 6 (6 hrs): Micronucleus Assay.

Theory:

  • Principle of micronucleus formation
  • Clastogenic and aneugenic agents
  • Applications in genotoxicity testing

Practical:

  • Sample preparation
  • Slide preparation and staining
  • Microscopic observation of micronuclei.

Assessment, Applications, and Feedback Certificate distribution on day 7th.

 
Learn From Experts

Our Trainers

Dr. Ganesh Narayanan
Dr. Ganesh Narayanan
Dr. N Ganesh, FICER, Dean R & D, Sanjeev Agrawal Global University Bhopal. EX- Consultant Oncogenetics, Head & Senior Scientist, Dept. of R...
Dr.Shikha
Dr.Shikha
DR. SHIKHA TIWARI Assistant Professor of Biotechnology| Head and Neck cancer research | +91-8305979899 [email protected], shikha...
Shivam Chaurasiya
Shivam Chaurasiya
SHIVAM CHAURASIYA M.Pharm (Pharmaceutics) | Diploma Clinical Research  ???? Bhopal, Madhya Pradesh, India ???? +91-7746814469 ...