Certificate Course in Mutation Research.

Mutation research is a core component of modern biological and biomedical sciences. This SWS certificate course provides theoretical foundations and hands-on laboratory training in cytotoxicity and genotoxicity assays such as Trypan Blue, MTT, and Micronucleus assays, aligned with NEP-2020 and industry requirements.

Duration

1 Week

Skill Level

Beginner

Starts From:

12/02/2026

Category

Science And Technology

Overview

The Certificate Course in Mutation Research is a focused, short-term program designed to introduce participants to the fundamental concepts and experimental approaches involved in the study of genetic mutations. The course covers key topics such as DNA damage, mutagens, mutation mechanisms, cell cycle alterations, and mutation-based assays, with strong emphasis on practical understanding and laboratory relevance. Through an integration of theory and hands-on learning, the program equips students with essential skills required for research in genetics, toxicology, cancer biology, and biomedical sciences. This course is ideal for students and early-career researchers seeking foundational training in mutation analysis and experimental genetics.

  • Introduce concepts of Cell, Mutation, Mutagenesis, and DNA damage.
  • Preparation of Hematological, Cytological & Histopathological Smear.
  • Explain principles of cytotoxicity and genotoxicity testing.
  • Provide hands-on training in Trypan Blue, MTT and Micronucleus assays.
  • Develop skills in data analysis and interpretation.
  • Enhance research competence and employability.
  • The participant will learn molecular basis and significance of mutations.
  • Trypan Blue & MT assay for cell viability.
  • Conduct MTT assay and calculate IC50 values.
  • Execute Micronucleus assay for genotoxicity assessment.
  • Analyze and interpret experimental mutation research data.


Our Trainers

Dr. Ganesh Narayanan

Dr. N Ganesh, FICER, Dean R & D, Sanjeev Agrawal Global University Bhopal.

EX- Consultant Oncogenetics, Head & Senior Scientist, Dept. of Research, Jawaharlal Nehru Cancer Hospital & Research Centre, since 1996.

He was awarded Ph.D. in Anatomy (Medicine) from Gandhi Medical College, Bhopal in 2002. He got trained from AIIMS New Delhi (Clinical & Molecular Genetics, Research Methodology), TATA Memorial Mumbai (Cancer Genetic), St. Johns Medical College Bangalore (Clinical Genetic), Kidwai Institute of Oncology Bangalore (Cancer Genetic), Kasturba Medical College Manipal (Radiation Biology), South West Foundation for Biomedical Research, San Antonio, USA in association with Anthropological Society of India (Molecular Genetic) & Beckman Coulter USA/India (Immunophenotyping).

He discovered active constituents against Skin Melanoma from Water Hyacinth (Jal Kumbhi). He also formulated herbal tea without sugar and tea leaves to reduce side effects of radiation and chemotherapy. I got patent for his formulation (Radiation Protector) extracted from Alpinia (Wild Zinger).

He am the first in the World to develop in-vivo Orthotopic Colon Tumor and metastasis Colon Tumor model in mice for targeted anti-cancer drug delivery.

He had an experience of working as Member Secretary of Institutional Ethics Committee for JNCHRC, Bhopal from 2000 and 2016. He is a Chairman of Institutional Ethics Committee of People’s Dental Academic since 2015.

He has completed 21 clinical and preclinical trials. Three ongoing. These projects were funded by ICMR, DST, DBT, CSIR, MPCOST, MPBTC, Elli Lilly, Cadila, Novartis, Johnson & Johnson, and Apothecaries etc.

He had published 134 scientific publications with a total impact factor of 210.11. He has presented more than 108 scientific papers & 157 posters at International & National Conference.   Received 89 best paper and 72 best poster awards. Invited as Guest Speaker/Resource Person 840.

He guided 62 Ph.D & 300 Post graduate Dissertation Thesis. He is a registered Ph.D. Guide in Genetics, Biotechnology, Zoology, Life Sciences, and Pharmacology & Medicinal Chemistry from 7 National Universities.

He has received numerous International & National awards including Humboldt Kolleg Young Scientist appreciation Award (2003 Manipal, Udipi, Karnataka), State Best Scientist (Cancer Research 2013), VISWA (BHEL Society) award for Scientific contribution to state and Nation 2012,  UGC sponsored Best visiting fellow Award in Pharmacology, Panjab University, Chandigarh, Punjab twice 2010 and 2013.  Received Kadimey Millat Award for his contribution in the field of Cancer genetic diagnosis and Cancer Research in MP & CG. Eminent Scientist Award for Orthotopic tumor model by Rajeev Gandhi Group of Institutions (2014), Best Social worker Award in the field of Cancer awareness at Bundelkhand Vigyan Mela by MPCOST (2014). “Visiting Fellow Award” for best visiting faculty in Pharmaceutical Sciences of Panjab University, Chandigarh, Punjab. (2015), Excellence of service in the field of Cancer at Mandla Vigyan Mela by MPCOST (2015).  “Award of Appreciation” for the contribution in the field of Cancer Awareness by Bhopal Mela Samiti (2016) and “Award of Appreciation” for tobacco cessation programme by People’s Group, Bhopal, (2017).  “Outstanding Achievement Award” for the extra ordinary contribution in serving the society by BHEL education society Bhopal (2018). “ Gaurav Samman” for the untiring contribution in the field of Cancer Awareness by Parakaram Jansevi Sansthaan, Jago Bharat Abhiyan and Jago Bharat Chatr Sangathan of India, (2018). Sr. Scientist Award – ACAEE -2018.  Outstanding Achievement Award (2019) by Utsav Mela Samiti, Bhopal for cancer awareness.  “You Make a Difference award” (2020) by JNCHRC, Bhopal for outstanding performance throughout the tenure of service of 23 years at JNCHRC, Bhopal.  Received “Award of Appreciation” for talk on “Cancer Awareness and Anticancer Diet Awareness:  Myth to Fact” organized by UIPS, Panjab University (2020).  Received "Healthcare Research Award for Genetic Oncologist of the Year" at “Asia Healthcare Summit & Awards 2020organized by Asia Today Research & Media.  MJF LION A V Mahajan (North Bhopal) Award for the outstanding services in the field of “Genetic diagnosis and anti cancer diet awareness” 2020.  India Star Book of Record Awarded “India Star Personality Award 2020for the appreciable work in the field of Anatomy. International Association of LIONS Club Awarded Dr.Ganesh for selfless services to the society 2020.  At Constitutional Club of India, New Delhi by National Achievers Recognition Forum received National award for Cancer Awareness 2021.  Institute of Scholars Research Excellence Award for the quality of work in academic and research, 2021. Outstanding work in the field of Cancer Genetics & Male Infertility awarded by; Intellectual Peoples Foundation, 2021.  Received Pride of India Award- 2022 in recognition of sterling Merit Excellent Performance and outstanding contribution for the progress of the nation & worldwide at Constitutional Club of India, New Delhi by National Achievers Recognition Forum, 1st December, 2022Received “Excellence in Oncogenetics Award” in recognition of sterling Merit Excellent Performance and Out-standing contribution since 1996 in Central India in the field of Clinical Genetics and Oncogenetics in Bangkok Thailand, on 8th & 9th December, 2022 at an International Conference organized by “PRISAL- Pharmaceutical Royal International Society” & Co-hosted by “TIPA- Thai Industrial Pharmacist association” on ‘Expanding Multi-Disciplinary Approaches for Healthcare & Well-being’ at Ambassador Hotel, Bangkok Thailand.

He is a life member in following societies:  Indian Society of Human Genetics, Bhopal Haematology Society, Fellow Member - FICER (International Congress of Environmental Research), Society for Biosafety of India, Indian Fertility Society & The Cytometry Society of India

I had organized “Cancer Awareness Camps” on a regular basis and so far completed  2023 “Cancer and Anticancer Awareness Talks & Camps” throughout Bhopal and nearby places like Vidisha, Khurai, Raisen, Sagar, Rewa, Hoshangabad, Itarsi, Chindwara, Seoni Binaganj, Guna, Mandi Bamora, Sehore, Indore, Chatterpur, Damoh, Khajraho, Piperiya, Shuhagpur, Sarangpur, Panchmari and other remote villages of Madhya Pradesh, Chattisgarh, Punjab, Haryana, Jharkhand, Bihar, Jammu & Kashmir, Tamil Nadu, Kerala, Maharashtra, Gujrat, Utter Pradesh, Telangna, Andhra Pradesh, Pudochery, Karnataka, Chandigarh .  The camps and talk were conducted Live and Virtual. He also conducts awareness talk on running trains and other public places.

He authored an International books on “Dermo Follicular Proliferation”- VDM Muller Publication, Germany & “Cytogenetic Variations in Bhopal Gas Tragedy Survivors” – Lambert Academic.

He am the only specialist in Diagnostic Clinical Genetics, Cancer Genetics, Genetic counselling in the entire Madhya Pradesh State and Chhattisgarh with special reference to Leukemia, Lymphoma, Multiple myeloma, Male infertility, Heritable diseases, Recurrent abortion, Sickle Cell Anaemia, Thalassemia.  

He designed Animal house (27 Nation Wide) and Biotechnology Lab (07 Nation Wide).

In addition to scientific background, Dr.Ganesh also received many more awards in Painting, Rangoli, Collage, Clay Modelling & Classical Dance.     

He visited Stockholm, Sweden to attend the investigator’s meeting for an International project Drug Trial on Locally Advanced Breast Cancer. Mauritius as a guest speaker in an “International conference on Environmental Research. He is also visited Dundee, Scotland to deliver a talk ‘Innovation in tumor biology and Role of biomarkers in time since death’.    

Dr.Shikha

DR. SHIKHA TIWARI

Assistant Professor of Biotechnology| Head and Neck cancer research |

+91-8305979899 [email protected],

[email protected] https://orcid.org/0000-0001-8871-9842 Bhopal, India

SUMMARY Biological Research Scientist with over 6 years of experience integrating molecular biology techniques and cancer research. Demonstrated ability to optimize laboratory operations and deliver comprehensive publications aligned with strategic objectives and translational approach, with a passion for advancing cancer treatment.

EXPERIENCE Assistant Professor Institute of Advanced Research, Gandhinagar 05/2024 - 11/2024 Gujarat Conduct academic lectures and practical sessions for Biotechnology Students. Explore the role of microRNA in oral cancer diagnosis and therapeutic advancements Women Scientist All India Institute of Medical Sciences Bhopal 08/2021 - 04/2024 Bhopal, India Established the MicroRNA dysregulation role in oral cancer development and progression. Successfully investigated the role of single nucleotide polymorphism in oral cancer predisposition. Optimized tissue culture conditions, resulting in a consistent 20% improvement in viability for cryopreserved cells. Provided critical input in team meetings, shaping the direction of research and subsequent studies. Senior Research Fellow CIAE 06/2019 - 07/2021 Bhopal, India Contributed to the development of a novel volatiles for the storage of horticulture commodity. Assisted in the validation of e-nose Prepared detailed method files for GC-MS and optimize VOCs absorption with SPME fibers.

EDUCATION Ph.D. Biological science and engineering Maulana Azad National Institute of Technology 03/2012 - 04/2016 Bhopal, India Master of Science in Biotechnology Krishna Institute of Medical Sciences University 07/2008 - 06/2010 Karad, India

LANGUAGES Hindi Native English Proficient

KEY ACHIEVEMENTS Publication in reputed SCI indexed journals Principal author of the research publications in peer reviewed journals. From the total 17 publications, 10 are published in SCI indexed journals. One book edited"Diagnostic, Prognostic, and Therapeutic Role of MicroRNAs in Head and Neck Cancer" Elsevier. Active member of American association of cancer research Describe what you did and the impact it had. Research Grant Recipient Secured a 47 lakhs research grant from ICMRDHR

SKILLS Cell Culture Protocols Technical SOPs Assay Development Transfection optimization Data analysis

COURSES 'R' workshop Received certification on R software for expression analysis and processing Online Course on Research Ethics Committee Completed a rigorous and informative course on making and implementation of ethical research committee

Shivam Chaurasiya

SHIVAM CHAURASIYA

M.Pharm (Pharmaceutics) | Diploma Clinical Research 

???? Bhopal, Madhya Pradesh, India
???? +91-7746814469

Professional Summary

Motivated Clinical Research and R&D professional with over 3 years of experience in hospital-based research, clinical genetics, and academic R&D coordination. Strong background in pharmaceutical research, clinical trials, laboratory techniques, and scientific writing, with multiple peer-reviewed publications.

Current Position

R&D Coordinator
Research & Development Cell,
Sanjeev Agrawal Global Educational (S.A.G.E.) University, Bhopal

Modules

Module 1 (4 hrs): Introduction to Cell Biology & Mutation Research

  • Definition and classification of mutations
  • Spontaneous vs induced mutations
  • Physical, chemical, and biological mutagens
  • DNA damage and repair mechanisms
  • Importance of mutation research in cancer biology and toxicology
  • Ethical and biosafety considerations

Module 2 (4 hrs): Cell Identification.

Differentiation between hematological, Cytological & Histopathological Smear/Section.

Practical:

  • Preparation of blood smear
  • Preparation buccal smear
  •  Preparation of Microbial smear
  • Staining (Hematological, Cytological & Histopathological Smear)

Module 3 (4 hrs): Cell Culture and Cytotoxicity Concepts

Theory:

  • Basics of cell culture techniques
  • Cell viability and cytotoxicity assays
  • Principles of dye exclusion methods

Practical:

  • Introduction to laboratory equipment
  • Aseptic handling of cells
  • Preparation of reagents

 

Module 4 (4 hrs): Trypan Blue Exclusion Assay

Theory

  • Principle of Trypan Blue Assay

Practical:

  • Cell counting using hemocytometer
  • Assessment of live and dead cells
  • Calculation of cell viability percentage
  • Data recording and interpretation

Module 5 (4 hrs): MTT Assay – Principle, Execution, and Data Analysis

Theory:

  • Principle of MTT assay
  • Role of mitochondrial activity in cell viability
  • Applications in cytotoxicity screening

Practical:

  • Preparation of MTT reagent
  • Treatment of cells with test compounds
  • Measurement of absorbance using ELISA reader
  • IC₅₀ determination
  • Graphical representation of results
  • Statistical interpretation
  • Limitations and precautions of MTT assay

 

 

 

Module 6 (6 hrs): Micronucleus Assay.

Theory:

  • Principle of micronucleus formation
  • Clastogenic and aneugenic agents
  • Applications in genotoxicity testing

Practical:

  • Sample preparation
  • Slide preparation and staining
  • Microscopic observation of micronuclei.

Assessment, Applications, and Feedback Certificate distribution on day 7th.

 


Outcomes

Certificate course in mutation research typically involve developing skills in interpreting genetic variants, understanding the causes and mechanisms of mutations, and applying this knowledge to specific fields like cancer research, Pharma-toxicology or plant breeding


Contact

Name: Dr. Ganesh Narayanan

Mobile No: 09826321616

Email: [email protected]


Course Image

This Premium course is included in plans

1000/-

Registration Closed

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